Skip to main content
. 2017 Jun 22;45(15):9108–9120. doi: 10.1093/nar/gkx537

Figure 5.

Figure 5.

Analyses of 5′-tRNA halves and td-piRNAs in BmNSun2-depleted BmN4 cells. (A) BmNSun2 mRNA from BmN4 cells treated with dsRNAs targeting Rluc (negative control) or BmNSun2 was quantified by qRT-PCR. Each data set represents the average of three independent experiments with bars showing the SD. (B) Total RNA from Rluc- or BmNSun2-depleted cells was subjected to denaturing PAGE and stained by SYBR Gold. Long exposure enabled clear observation of the piRNA bands. (C) Total RNA from Rluc- or BmNSun2-depleted cells was subjected to northern blot targeting the Bombyx 5S rRNA. (D) Total RNA from Rluc- or BmNSun2-depleted cells was subjected to Northern blot targeting the 5′-part of mature tRNAAspGUC and tRNAHisGUG. Because the 5′-part of the tRNA was targeted, 5′-half and td-piRNA, as well as mature tRNA, were all detected. We failed to detect td-piRHisGUG due to a lack of sensitivity. The northern blot bands were quantified and shown as relative abundance in the right graph. Abundances in Rluc-depleted cells were set as 1, and the averages of three independent experiments with bars showing the SD are shown. (E) The 5′-halves and td-piRNAs in Rluc- or BmNSun2-depleted cells were quantified by TaqMan qRT-PCR. The amounts in Rluc-depleted cells were set as 1, and relative amounts are indicated. Averages of three independent experiments with SD values are shown.