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. 2017 Jun 28;45(15):e141. doi: 10.1093/nar/gkx568

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Estimation of the hybridization efficiency of single probes. (A) The effect of varying the number of probes. The histograms of the number of spots detected per cell are plotted for 1-probe (blue) and 5-probe (white) FISH. (B) Spot intensity vs. probe number. The mean spot intensity increases linearly with the number of probes as expected from the binomial distribution. (C) Spot number versus probe number. The mean number of spots detected per cell (y) increases with the number of probes (x). The fit model is y = N(1 − (1 − p)^x) where N is the true copy number, and p is the hybridization rate for a single probe. p is extracted to be 53%.