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. 2017 Jul 14;45(15):8978–8992. doi: 10.1093/nar/gkx612

Figure 1.

Figure 1.

The role of cas genes in de novo spacer acquisition. (A) Organization of CRISPR–Cas Type I-A and Type III-B modules in Sulfolobus islandicus REY15A. (B) Detection of spacer acquisition at CRISPR loci 1 and 2 in csa3a-overexpressing strains. Each lane represents an S. islandicus E233S strain (wt carrying the empty vector pSeSD; wt and mutants of the indicated genes carrying the csa3a-overexpression plasmid, pCsa3a). The PCR products of the leader-proximal regions of CRISPR loci 1 and 2 from these strains were separated on a 1.5% agarose gel. The parental and expanded bands are indicated by arrows. This result represents three independent spacer acquisition analyses.