Table 1. Analysis of the protospacers.
| Protospacer | wt::csa3a* | Δcas6::csa3a | Δcas3cmr2αcmr2β::csa3a |
|---|---|---|---|
| Total | 160 | 218 | 211 |
| genomic DNA | 11 (6.9%) | 159 (72.9%) | 157 (74.4%) |
| plasmid | 149 (93.1%) | 59 (27.1%) | 54 (25.6%) |
| Forward strand | 88 (55%) | 112 (51.4%) | 111 (52.6%) |
| Reverse strand | 72 (45%) | 106 (48.6%) | 100 (47.4%) |
| PAM sequence | |||
| CCN | 115 (71.9%) | 190 (87.2%) | 188 (89.1%) |
| CCA | 47 (29.4%) | 68 (31.2%) | 66 (31.3%) |
| CCT | 32 (20%) | 90 (41.3%) | 83 (39.3%) |
| CCG | 21 (13.1%) | 18 (8.3%) | 20 (9.5%) |
| CCC | 15 (9.4%) | 14 (6.4%) | 19 (9.0%) |
| No PAM | 45 (28.1%) | 28 (12.8%) | 23 (10.9%) |
| Adapted spacer units | |||
| Single | 126 | 57 | 76 |
| two | 15 | 36 | 34 |
| three | 0 | 14 | 16 |
| four | 1 | 9 | 2 |
| five | 1 | 1 | |
| six | 1 | 1 | |
The numbers of protospacers matching the expression plasmid and genomic DNA, as well as the protospacers having an adjacent PAM sequences (5′-PAM-protospacer-3′), are given. Percentages are included in brackets. Cells were cultured in SCVy non-inducible medium. *: data from reference (19).