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. 2017 Jul 21;45(15):9019–9029. doi: 10.1093/nar/gkx620

Figure 6.

Figure 6.

m1G9 formation by Trm10 is associated with a single basic ionization. Single turnover kobs (min−1) for tRNAGly methylation were measured at saturating enzyme concentrations at varied pH for (A) ScTrm10 WT and D210 variants; (B) ScTrm10 D100N and E111A variants and (C) hTRMT10A WT and D210N variant. WT enzymes are represented by open circles and solid fits and are repeated in all panels for better comparison with each variant. Note the difference in the Y-axis scale (all rates are reported as min−1) for each variant The fits correspond to the best fit to Equation (4) corresponding to a single ionization for each reaction and yield the following pKa values: ScTrm10 WT (pKa = 8.1 ± 0.1); ScTrm10 D210A (pKa = 7.1 ± 0.1); ScTrm10 D210K (pKa = 7.2 ± 0.1); ScTrm10 D100N (pKa = 6.9 ± 0.1); ScTrm10 E111A (pKa = 8.0 ± 0.1); hTRMT10A WT (pKa = 7.9 ± 0.2); hTRMT10A D210N (pKa = 7.3 ± 0.1).