Schematic of the experimental setup. The iPerfusion system comprises an actuated reservoir to control the applied pressure, a thermal flow sensor to measure the flow rate from the reservoir, and a differential pressure sensor to measure the pressure in the eye with respect to the bath. For the single cannula experiments, the eye was cannulated with one glass needle connected to iPerfusion. For the double cannula experiments, the eye was cannulated with two glass needles, connecting it to both the iPerfusion system and a syringe pump, used to impose a known pressure-independent flow.