Figure 5.

‘Step by Step’ cyclization of l-DNA. (A) Schematic view of the method. (B) Yields of c-DNA₆₆ by this method (S-b-S). The cumulative concentrations of l-DNA₆₆ (the concentrations of all the substrates added to the mixture) = 10 μM (left) and 30 μM (right). Details of the procedure should be referred to the main text. For the purpose of comparison, the results of conventional cyclization in 1 × buffer and single-addition cyclization in 0.05 × buffer are also shown. The selectivity for the formation of c-DNA₆₆ is presented below the corresponding band.