Table 2. Kinetic parameters for 5′-dRP removal by Pol β in DNA and NCPs.

Substrate	k app (min-1)	Ratio (DNA/NCP)
DNA-dRP-O (+10)	0.009 ± 0.001
NCP-dRP-O (+10)	0.021 ± 0.004	0.43 ± 0.03
DNA-dRP-O (-35)	0.21 ± 0.01
NCP-dRP-O (-35)	0.28 ± 0.003	0.75 ± 0.03
DNA-dRP-I (-49)	0.09 ± 0.002
NCP-dRP-I (-49)	0.07 ± 0.004	1.3 ± 0.04
DNA-dRP (-60)	0.01 ± 0.001
NCP-dRP (-60)	0.16 ± 0.01	0.06 ± 0.002

Data represent the average of three independent experiments ± SD with the error in the ratio calculated using standard propagation of error with standard deviation procedures. Data correspond to plots shown in Figures 3B and 4 and are expressed as initial rate/enzyme concentration.