Table 3. Kinetic parameters for 5′-dRP removal by the 8 kDa domain of Pol β in free DNA and NCPs.
| Substrate | k app (min-1) | Ratio (DNA/NCP) |
|---|---|---|
| DNA-dRP-O (+10) | 0.015 ± 0.002 | |
| NCP-dRP-O (+10) | 0.016 ± 0.001 | 0.94 ± 0.07 |
| DNA-dRP-O (-35) | 0.24 ± 0.01 | |
| NCP-dRP-O (-35) | 0.06 ± 0.005 | 4.0 ± 0.17 |
| DNA-dRP-I (-49) | 0.02 ± 0.001 | |
| NCP-dRP-I (-49) | 0.006 ± 0.005 | 3.3 ± 0.11 |
| DNA-dRP (-60) | 0.05 ± 0.002 | |
| NCP-dRP (-60) | 0.14 ± 0.01 | 0.36 ± 0.02 |
Data represent the average of three independent experiments ± SD for all except for dRP-O (+10), which were performed in duplicates for DNA and NCP, in which case the error in the fit is shown. Data correspond to plots shown in Figures 3B and 4 and are expressed as initial rate/enzyme concentration.