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. 2017 Jul 26;45(15):9046–9058. doi: 10.1093/nar/gkx633

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — RNA-dependent DNA polymerase activity shown by WT HsPrimPol and Y89R variant. (A) Names and sequences of oligonucleotides used as template/primer hybrids to measure primer-dependent dNTP incorporation by HsPrimPol; RNA sequences are indicated in italics; ‘*’ indicates the radiolabeled oligonucleotide used as primer. (B) Polymerization reactions by WT or Y89R variant HsPrimPol was evaluated using the indicated combinations of dNTPs (100 μM each) and either DNA (T13T/Spc1) or RNA (T13U/Spc1) templates. As shown by electrophoretic analysis, primers (15-mer) elongated in 2, 4, 6 or 13 residues represent the expected products in the presence of either dATP, dATP+dGTP, dATP+dGTP+dTTP, or the four dNTPs, respectively.