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Y89R can form a preternary complex on ssRNA. EMSA analysis and mobility of the preternary complex. WT and Y89R proteins (450 nM) were incubated with either GTCC or GUCC templates (1 μM) and labeled dGTP to form a preternary complex. Cartoons represent preternary complex formation between HsPrimPol (gray), a DNA template (GTCC) or an RNA template (GUCC), and dGTP, which occupies the 3′ elongation site (white circle) in HsPrimPol.
