Table 1. Kinetic constants for dATP incorporation by WT and Y89R PrimPol^a.

PrimPol	K M (μM)	k cat (min−1)	k cat/ K M (μM−1 min−1)
WT	3.59±0.41	0.23±0.01	0.07±0.01
Y89R	2.49±0.52	0.22±0.01	0.09±0.02

^aExperiments were carried out as described in Materials and Methods. R² values of the nonlinear regression analysis from data obtained in kinetic assays using the WT HsPrimPol and Y89R were 0.985 and 0.964, respectively.