Table 1. Estimated error frequency for model gene assemblies.

Error type	Q5	KAPA2G Robust
Mismatches	0.2131 ± 0.0019	7.1388 ± 0.0121
Single base deletions	2.0121 ± 0.0062	2.1891 ± 0.008
Single base insertions	0.0747 ± 0.0011	0.0816 ± 0.0014
Multiple base deletions	0.2326 ± 0.002	0.2342 ± 0.0029
Multiple base insertions	0.0014 ± 2e-04	0.0083 ± 4e-04

Here, we averaged the errors/kb for both five-oligo assemblies using Q5 and KAPA2G Robust polymerases and their technical replicates across each error type (errors are standard error of the mean). We see that all error subtypes are similar except for mismatches.