Table 3. Precision of the PowerChek MERS (upE and ORF1a) real-time PCR Kit.
| Target | Concentration (copies/µL) | Mean Ct (% coefficient of variation) | |||||
|---|---|---|---|---|---|---|---|
| Reproducibility | Repeatability | Lot-to-lot variation | |||||
| Site 1 | Site 2 | Lot 1 | Lot 2 | Lot 3 | |||
| upE | 50 | 34.40 (2.10) | 34.40 (1.32) | 34.74 (2.11) | 34.40 (2.10) | 34.45 (3.36) | 34.40 (2.10) |
| 150 | 32.68 (0.89) | 32.85 (1.19) | 32.92 (1.06) | 32.68 (0.89) | 32.82 (0.97) | 32.68 (0.89) | |
| 300 | 31.91 (0.54) | 31.99 (0.88) | 31.97 (0.77) | 31.91 (0.54) | 31.98 (0.68) | 31.91 (0.54) | |
| ORF1a | 50 | 33.93 (1.30) | 33.90 (0.86) | 34.07 (1.53) | 33.93 (1.30) | 34.14 (1.34) | 34.04 (1.21) |
| 150 | 32.16 (0.79) | 32.11 (0.63) | 32.23 (0.77) | 32.16 (0.79) | 32.31 (0.77) | 32.27 (0.49) | |
| 300 | 30.88 (0.62) | 30.78 (0.62) | 30.91 (0.54) | 30.88 (0.62) | 30.85 (1.15) | 30.98 (0.70) | |
Abbreviation: Ct, Cycle threshold.