Table 4. Comparison of the PowerChek MERS (upE and ORF1a) real-time PCR Kit and the sequencing assay for the detection of MERS-CoV.

Detection method	Result	Subject characteristics		Total
		Symptomatic MERS-confirmed patients	Asymptomatic MERS-negative individuals
PowerChek MERS (upE and ORF1a) real-time PCR Kit	Positive	50	0	50
	Negative	0	50	50
RdRp and N gene sequencing assay	Positive	50	0	50
	Negative	0	50	50
	Total	50	50	100

Positive percent agreement, 100% (91.1–100%).

Negative percent agreement, 100% (91.1–100%).

Kappa coefficient, 1.00.

Abbreviations: MERS, Middle East respiratory syndrome; RdRp, RNA-dependent RNA polymerase.