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. 2017 Aug 15;27(9):1128–1141. doi: 10.1038/cr.2017.102

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Copyright © 2017 The Author(s)

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D53 represses the transcriptional activation activity of IPA1. (A) Transcriptional activity assay in tobacco leaves, showing that IPA1 promotes the expression of the reporter gene Luciferase (LUC) driven by the GTAC-containing promoter. A. tumefaciens transformed with Pro35S:IPA1-MYC, Pro35S:GFP, Pro35S:GUS, and ProGTAC:LUC were mixed and injected into tobacco leaves. D-luciferin was used as the substrate of LUC. (B) Statistical analysis of (A). Values are means ± SD (n = 3). The asterisks represent significant difference determined by Student's t test. ^**P < 0.01. (C) Transcriptional activity assay in tobacco, showing that D53 represses the transcriptional activation activity of IPA1. A. tumefaciens transformed with Pro35S:IPA1-MYC, Pro35S:GFP, ProGTAC:LUC, Pro35S:GUS, and Pro35S:FLAG-D53 were mixed and injected into tobacco leaves. D-luciferin was used as the substrate of LUC. (D) Statistical analysis of (C). Values are means ± SD (n = 3). Different letters at top of each column indicate a significant difference at P < 0.05 determined by Tukey's HSD test. (E) Protein levels in different infiltration combinations in (C). D53 was detected by rabbit polyclonal antibodies anti-D53, GFP by mouse monoclonal antibody anti-GFP, and IPA1 was detected by rabbit polyclonal antibodies anti-IPA1. Ponceau S staining was used as loading control. See also Supplementary information, Figure S5.