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. 2017 Aug 15;27(9):1128–1141. doi: 10.1038/cr.2017.102

Figure 7.

Figure 7

Overexpression of miRNA156 compromises SL response. (A) Gross morphologies of wild-type and miRNA156 overexpression (miR156OE) plants with or without rac-GR24 treatment. Seedlings were treated with 1 μM rac-GR24 (+) or mock (–). Bar = 5 cm. (B) Statistical analysis of tiller number in (A). Values are means ± sem (n = 5). The asterisks represent significant difference determined by Student's t test. ***P < 0.001; ns, no significant difference. (C) Overexpression of miRNA156 disrupts SL-induced D53 transcription after rac-GR24 treatment. Values are means ± sem (n = 3). Statistical differences between mock and treatment at the same time points were determined by Student's t test. **P < 0.01; ns, no significant difference. (D) Gross morphologies of d53, ipa1-1D, and d53 ipa1-1D double mutant plants. Bar = 20 cm. (E) Statistical analysis of (D). Values are means ± sem (n = 8). Different letters at top of each column indicate a significant difference at P < 0.05 determined by Tukey's HSD test. (F) A proposed model of the IPA1-mediated SL signaling pathway. In the absence of SLs, the D53 protein binds to IPA1, and together with TPL/TPR proteins represses the transcriptional activity of IPA1. In the presence of SLs, perception of SL leads to degradation of D53 by the proteasome system, which in turn releases the repression of IPA1-regulated gene expression and leads to SL response.