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Schematic for identifying phosphoproteins and phosphopeptides via liquid chromatography tandem mass spectrometry. A, Workflow of sample preparation. Mycelial proteins were extracted and trypsin-digested. Total proteins (10 mg) were used for trypsin digestion and the tryptic peptides were subjected to SCX fractionation and IMAC phosphopeptide enrichment. B, A neutral loss triggered ETD strategy for data acquisition using an amaZon speed ETD mass spectrometer. C, Process of data analysis.
