Fig. 1.

Upregulation of Salmonella YdcR at 18 hpi. A, The spectral counts of YdcR and two SPI-2 effectors (PipB, PipB2) derived from three independent LC-MS/MS measurements. Intracellular Salmonella was isolated from infected host epithelial cells at various time points (1 h, 6 h, and 18 h post-infection) and subjected to LC-MS/MS analyses together with bacteria cultured in LB media and those incubated further in HBSS prior to invasion (the extracellular population). These data were obtained from our previous studies (5, 6) and the original proteomic dataset can be found at http://iai.asm.org/content/83/7/2897/suppl/DCSupplemental and http://pubs.acs.org/doi/suppl/10.1021/acs.jproteome.6b00793. Asterisks indicate significant differences (*, p < 0.05; **, p < 0.01). B, Representative immunoblotting data of YdcR (and DnaK as a loading control) by using host cells infected by the Salmonella strain chromosomally expressing 3×FLAG-tagged YdcR.