Determination of YdcR-binding sites in the promoter region of srfN by EMSA.
A, Various DNA fragments covering different regions upstream of srfN were tested in the EMSA experiments. The numbers represent the positions of the fragments with respect to the translation start site. B, EMSAs were performed by incubating serial concentrations of purified YdcR (ranging from 0 to 400 nm) with the DNA fragments B1, B2, B3, B4, and B5 prior to electrophoretic separation.