Fig. 2.
Assessment of the impact of normalization methods on the accuracy of KARP. (A) Formula for the sensitivity coefficient, SC. (B–D) The P31/Fuj cell line was treated with the named compounds and their viability measured after 48 h using the Guava Viacount assay. The K-score (K) for the named kinases were calculated as shown on the chart headings and compared with the IC50 of inhibition of cell viability as a function of treatment with the inhibitors shown (C) or to the SC (D). K-score values represent the mean of four independent experiments, each measured in duplicate.