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. 2017 Sep 5;19(10):805–816. doi: 10.1016/j.neo.2017.07.003

Figure 2.

Figure 2

In vitro CCSP-2–targeted probe binding study. (A) Western blot showing overexpression of CCSP-2 in HCT116 cells transfected with a CCSP-2–encoding plasmid. Arrowheads denote the positions of the full-length 85-kDa CCSP-2 protein and 55-kDa CCSP-2–derived peptide detected in the lysates of CCSP-2–transfected cells. (B) CCSP-2–overexpressing cells (HCT116/CCSP-2) and corresponding control cells (HCT116) were incubated with CCSP-2–targeted probe. In vitro confocal microscopy of the cell cultures confirmed binding of the CCSP-2–targeted probe (red) to CCSP-2–overexpressing cells, whereas control cells showed no CCSP-2 staining. Nuclei were counterstained with DAPI (blue). Original magnification, 400×. Scale bar: 50 μm.