FIG 3 .

Hepatitis A infection in Tim1^−/− Ifnar1^−/− (n = 4), Tim4^−/− Ifnar1^−/− (n = 2), and Ifnar1^−/− (n = 4) mice inoculated intravenously (i.v.) with ∼10⁸ genome equivalents (GE) of fifth mouse-passage HM175 virus (mp5 unfractionated liver homogenate). (A) Fecal shedding of HAV by Tim1^−/− Ifnar1^−/−and Tim4^−/− Ifnar1^−/−double-knockout mice was similar to single Ifnar1^−/− knockouts, but significantly elevated in Tim1^−/− Ifnar1^−/− versus Ifnar1^−/− mice on day 7 postinfection (P < 0.01). (B) Intrahepatic HAV RNA abundance at day 14 (d14) postinfection. (C) Serum ALT on days 7 and 14 postinfection. Bar = mean. (D) H&E-stained liver sections from mock-infected Tim1^−/− Ifnar1^−/− mice, mp5 HM175 virus-infected Tim1^−/− Ifnar1^−/− mice, and Ifnar1^−/− mice. Virus-infected Tim1^−/− Ifnar1^−/− and Ifnar1^−/− livers show diffuse small inflammatory cell infiltrates with apoptotic hepatocytes (white arrows) scattered throughout the parenchyma. Only minimal numbers of periportal lymphocytes are evident in mock-infected liver tissue. Bar, 100 µm.