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4,4′-Diapophytoene accumulation after NP16 treatment. (A) Comparison of carotenoid components among strain COL, COL-ΔcrtN, COL-ΔcrtN-pOS1hrtAB-crtN, or COL-ΔcrtN-pOS1hrtAB-crtN plus 10 μM hemin (inducer), or COL treated with NP16. Plasmid pOShrtAB-crtN has a hemin-inducible promoter. (B) Comparison of the pigmentation of the wild-type COL strain, crtN deletion strain, and crtN complemented strain.
