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. 2017 Sep 5;8(5):e01284-17. doi: 10.1128/mBio.01284-17

FIG 4 .

FIG 4 

Hog1-MAPK is involved in tolerance to heat-induced acid stress in M. robertsii. (A) Vacuolar pH values in hyphae grown at the optimal temperature and after 10 min at 35°C. Values with different letters are significantly different (one-way ANOVA, P < 0.05). (B) Heat-treated hyphae (35°C for 10 min) stained with BCECF-AM. Note that more red spots are visible in the Hog1-MAPK (ΔMero-Hog1) deletion mutant, suggesting a pH higher than that of the WT strain. Bars, 10 μm. Presented are typical pictures representative of five repeats. (C) pH values in supernatants of heat-treated WT and ΔMero-Hog1 mutant cultures. (D) Growth curves of WT and ΔMero-Hog1 mutant hyphae on buffered PDA plates (pH 5) at the optimal temperature (26°C). The growth rates differed significantly (t test, P < 0.01) at ≥4 days postinoculation. Bars, 10 mm. Pictures were taken 14 days postinoculation. Presented are typical pictures representative of three repeats.