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Heterologous expression of vio2ta16 challenges the assumption that more closely related hosts perform better. Heterologous violacein production by four proteobacterial host strains was determined by extraction and HPLC quantification after 48 h of liquid culture in LB medium at 18 or 30°C. The data plotted are the mean ± standard error from two independent experiments (total n = 6). Plate images were taken after 48 h of growth at 30°C.
