Figure 2.

siRNA against Notch 1 was successfully transfected into clear cell renal cell carcinoma 786-O cells and inhibited the mRNA and protein expression of Notch 1. (A) A random sequence marked with fluorescence was transfected into 786-O cells as the parallel. Positive cells were detected under an inverted fluorescence microscope and total 786-O cells were observed at the same visual field of the microscope (magnification, ×200). At 24 h post-transfection with Notch 1-specific siRNA and negative control siRNA, (B) mRNA and (C) protein expression of Notch 1 in 786-O cells was detected by reverse transcription-polymerase chain reaction and western blot analysis, respectively. The mRNA and protein expression of Notch 1 in 786-O cells was dose-dependently inhibited by increasing concentrations of Notch 1-specific-siRNA (40, 80 and 120 nmol/l) compared with the control. *P<0.05, **P<0.01 vs. control. siRNA, small interfering RNA.