Figure 4.

Inhibition of Notch 1 expression with Notch 1-specific-siRNA suppressed the activity of the Akt/mTOR signaling pathway in clear cell renal cell carcinoma 786-O cells. Expression of key components in the Akt/mTOR signaling pathway was evaluated by western blot analysis. (A) Inhibition of Notch 1 expression had no effect on total Akt expression, but inhibited the expression of p-Akt, p-mTOR and p-P70S6K in a dose-dependent manner. Quantitative determination of (B) Akt and p-Akt, and (C) p-mTOR and p-P70S6K expression following normalization by β-actin. Data are presented as the mean ± standard deviation of three independent western blot assays and presented as column diagrams. *P<0.05, **P<0.01 vs. control. siRNA, small interfering RNA; mTOR, mechanistic target of rapamycin; p-, phosphorylated-.