Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Bromodomain containing 4 (BRD4) was a direct target of hsa-miR-599. (A) Sequence alignment of human hsa-miR-599 with 3′-untranslated region (3′-UTR) of BRD4 predicted by TargetScan. (B and C) BRD4 protein expression in breast cancer cell lines was determined through western blot analysis at 72 h post-transfection. β-actin was used as a control (*P<0.05). Luciferase assay results for (D) MCF-7 and (E) MDA-MB-231 cells showed that hsa-miR-599 upregulation decreased luciferase activity.
