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. 2017 Jul 21;14(3):3363–3370. doi: 10.3892/ol.2017.6652

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Copyright: © Gnanamony et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Role of chronic radiation on angiogenesis and invasion. (A) Angiogenesis was studied using the in vitro endothelial cell tube formation assay. Representative images illustrate decreased angiogenesis in human umbilical vein endothelial cells treated with conditioned medium from chronic radiation cells. (B) In vitro invasion was studied using the Oris Pro 96 well invasion assay kit. (C) After 48 h, invasion capability was decreased significantly in the SK-N-BE (2) and NB-1691 cells treated with chronic radiation compared with controls (*P=0.003).