Table 1.

Master mix preparation

	Column A	Column B	Column C	Column D	Column E
	[Stock]	[Final] in 100 μL well	[Final] in 95 μL	Volume needed for 1 well (μL)	Total volume needed in master mix (μL)
2× Adenylation assay buffer	–	1×	1×	50	125
Water	–	–	−	22.9	70.3
GrsA-A	1 μM	5 nM	5.25 nM	0.53	1.3
IP	40 U/mL	0.04 U	0.042 U	1.05	2.6
PNP	100 U/mL	0.1 U	0.105 U	1.05	2.6
MesG	1 mM	100 μM	105 μM	5.25	13.2
TCEP	100 mM	1 mM	1.05 mM	1.05	2.6
Hydroxylamine	2 M	150 mM	157.5 mM	7.7	19.3
ATP	100 mM	5 mM	5.25 mM	5.3	13.1