TABLE 2 .
Activities of promoter reporter fusions measured in shake flasks and in static cultures
| Reporter fusion | β-Galactosidase activity (mean ± SD)a |
|||
|---|---|---|---|---|
| Shake flask | Shake flask + NO | Static | Static + 5 mM nitrate | |
| hmp-lacZ | 391 ± 12 | 824 ± 12 | 895 ± 40 | 1,205 ± 109 |
| norC-lacZ | 91 ± 5 | 329 ± 17 | 179 ± 5 | 2,103 ± 140 |
a
Liquid cultures were grown in shake flasks to mid-log phase or for 72 h in static 6-cm-diameter petri dishes (the same conditions used for biofilm assays). For NO-treatment, shake flask cultures were amended with 50 μM spermine NONOate 60 min prior to sampling. β-Galactosidase was assayed according to Miller (69). Means and standard deviations were calculated from results of triplicate assays.