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. 2017 Jul 15;14(3):3503–3509. doi: 10.3892/ol.2017.6586

Figure 2.

Figure 2.

(A) NAC suppressed teroxirone-induced ROS. Cells were seeded onto 12-well plates (3×105 cells/well). Following 24 h for allowing complete adherence, the cells were pretreated with either NAC (10 µM) for 1 h (+) or the vehicle control (DMSO) (−), followed by treatment with teroxirone (2 µM) (+) or 0.2% DMSO as the vehicle control (−) for 18 h. Following treatment, the trypsinized cells were evaluated using flow cytometry, with DCFH-DA as a fluorescent oxidation-sensitive probe. (B) Cell and cell viability determination by teroxirone in A549 and H460 cells. Cells were seeded onto 6-well plates (3×105 cells/well). Following 24 h to allow for complete adherence, the cells were pretreated with either NAC (10 µM) for 1 h (+) or the vehicle control (DMSO) (−), followed by treatment with teroxirone (2 µM) (+) or 0.2% DMSO vehicle control (−) for 24 h. The trypsinized cells were counted using a trypsin-exclusion assay. *P<0.05 and **P<0.01 vs. DMSO control. DCFH-DA, dichlorodihydrofluorescein diacetate; DMSO, dimethyl sulfoxide; ROS, reactive oxygen species; NAC, N-acetylcysteine.