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. 2017 Jul 15;14(3):3566–3572. doi: 10.3892/ol.2017.6573

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Overexpression of miR-7 induces autophagy in HepG2 cells. (A) Effect of miR-7 on autophagy marker LC3 and p62 with or without treatment with CQ. (B) Quantification of the band density of LC3-II and p62. (C) The autophagosome as demonstrated by LC3 immunofluorescent staining. Arrows indicate the autophagosome dots. Scale bar, 25 µm. *P<0.05; n=5 in each group. LC3, 1A/1B-light chain 3; LC3-II, LC3-phosphatidylethanolamine conjugate; miR-7, microRNA-7; CQ, chloroquine; NC, negative control.