Figure 2.

Effects of the miR-383-5p expression level on proliferation 48 h after transient transfection with miR-383-5p mimic (or control mimic) in lung adenocarcinoma cells. (A) Validation of miR-383-5p expression level in A549 and H1299 cells using the reverse transcription-quantitative polymerase chain reaction. (B) [³H]thymidine incorporation assays were performed to examine cell proliferation 48 h after transfection. (C) Frequencies of cells at various stages of the cell cycle. (D) The proportion of apoptotic cells (Annexin V-fluorescein isothiocyanate, positive; propidium iodide, negative). Data are expressed as the mean ± standard deviation. n=5. miR, microRNA.