Figure 3.

miR-383-5p directly targets CIP2A by binding to its 3′UTR. (A) Immunohistochemistry demonstrated an inverse association between the expression levels of miR-383-5p and CIP2A in LAC tissue samples (scale bars, 100 µm). (B) Western blot analysis of CIP2A protein expression levels in LAC cells transfected with miR-383-5p mimic (or control mimic). (C) miR-383-5p and its putative binding sequence in the 3′UTR of CIP2A (lines indicate matching base pairs and crosses represent non-matching base pairs). (D) Dual-luciferase reporter analysis was performed in LAC cells that were co-transfected with miR-383-5p mimic (or control mimic) and reporter vectors carrying CIP2A 3′UTR with wild-type compared with mutated miR-383-5p response element. Data are expressed as the mean ± standard deviation. n=5. miR, microRNA; UTR, untranslated region; CIP2A, cancerous inhibitor of protein phosphatase 2A; LAC, lung adenocarcinoma; WT, wild-type; Mut, mutant.