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Ad-REIC treatment downregulates the expression of CD147 in human bladder cancer KK47 cells. The overexpression of REIC/Dkk-3 was induced by the Ad-REIC agent at 100 MOI and the expression level of the indicated proteins was analyzed by western blotting. β-actin was used as a loading control. REIC/Dkk3, reduced expression in immortalized cells/Dickkopf-3; CD147, cluster of differentiation 147; Ad-REIC, adenoviral vector encoding the human REIC/Dkk3 gene.
