(a) Zeta potential of different bioconjugates. (b) Typical
absorbance
and emission spectra of cHSA-PEO-TPP-Ru, where characteristic peaks
of the Ru complexes are preserved in cHSA-PEO-TPP-Ru. (c) Comparison
of the photostability of the Ru1 and cHSA-PEO-TPP-Ru based on the
absorbance decay under continuous irradiation over extended time periods.
(d) 1O2 production yield of cHSA-PEO-TPP-Ru
and bare Ru complex (Ru1), as obtained from the photobleaching of
the characteristic absorption peak @380 nm of ABDA (100 μM)
during irradiation with 470 nm LED light (∼20 mW/cm2, 5 min) in PBS (1×, pH 7.4) based on the same optical density
in their first absorption peak. (e) Steady state emission spectra
(λex = 460 nm) of Ru1 complex in water (red), Ru1
complex in simulated body fluid (orange), cHSA-PEO-TPP-Ru in water
(blue), cHSA-PEO-TPP-Ru in simulated body fluid (green), and Ru(bpy)3 (black) as reference at same optical density. (f) Emission
lifetime experiments upon excitation at 460 nm. For the compounds,
the same color code is used and an artifact region between 1.2 and
1.35 μs has been removed.