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. 2017 Jun 30;14(3):3051–3056. doi: 10.3892/ol.2017.6500

Figure 1.

Figure 1.

SPOPL protein and mRNA expression in established cell lines and paired clinical MB samples. (A) SPOPL protein expression in NHA and cultured MB Daoy, D283 and D341 cells. (B) SPOPL mRNA expression was quantified by RT-qPCR. The expression levels were normalized for GAPDH. Data are presented as the mean ± standard deviation. (C) SPOPL protein expression in each of the primary MB T and N samples based on Western blotting. (D) The average tumor/normal (T/N) ratios of SPOPL expression were quantified by RT-qPCR. The expression levels were normalized for GAPDH. Data are presented as the mean ± standard deviation. SPOPL, Speckle-type POZ protein-like; NHA, normal human astrocyte; MB, medulloblastoma; N, normal human cerebellum sample; T, tumor sample; RT-qPCR, quantitative reverse transcription polymerase chain reaction.