Figure 3.

Effect of etanercept treatment on NK cells, T cells and metastatic nodules in a mouse tumor model. Balb/c mice were injected with or without C26 colon cancer cells, and with or without etanercept or human IgG control as indicated. The following parameters were assayed following 14 days: (A) NK cell count in the blood (top) and spleen (bottom); (B) percentage of NK cell subsets defined based on CD27 and CD11b expression in the blood (top) and spleen (bottom); (C) CD3⁺CD4⁺ and CD3⁺CD8⁺ cell counts in the blood (top) and spleen (bottom); and (D) number of metastatic lung surface nodules. Data are presented as the mean ± standard error of the mean (n=8 animals). *P<0.05, **P<0.01, ***P<0.001. CD, cluster of differentiation; NK, natural killer; IgG, Immunoglobulin G.