Table I.
Antibodies used.
| A, Antibodies used for FACS | ||
|---|---|---|
| Fluorescent antibody for FACS | Cat. no. | Volume, µl |
| FITC anti-mouse CD3 | 561798 | 1 |
| PE anti-mouse CD335 (NKp46) | 560757 | 1 |
| APC-Cy7 rat anti-mouse CD11b | 557657 | 1 |
| APC hamster anti-mouse CD27 | 560691 | 1 |
| PE-Cy7 rat anti-mouse Ly6g and | 552985 | 1 |
| Ly6c (Gr1) | ||
| APC rat anti-mouse CD4 | 553051 | 1 |
| PE anti-mouse CD8a | 553032 | 1 |
| PE-Cy7 rat anti-mouse CD19 | 552854 | 1 |
| B, Isotype controls | ||
| Isotype control | Cat. no. | Volume, µl |
| FITC rat IgG2bκ | 556923 | 1 |
| PE rat IgG2aκ | 553930 | 1 |
| APC-Cy7 rat IgG2bκ | 552773 | 1 |
| APC hamster IgG1κ | 553974 | 1 |
| PE-Cy7 rat IgG2bκ | 552849 | 1 |
| APC rat IgG2aκ | 553932 | 1 |
| PE rat IgG2aκ | 353930 | 1 |
| PE-Cy7 rat IgG2aκ | 552784 | 1 |
All antibodies were purchased from BD Biosciences. FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; CD, cluster of differentiation; APC, allophycocyanin; PE, phycoerythrin; NK, natural killer; Cy, cyanine; IgG, immunoglobulin G.