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. 2017 Jul 5;14(3):2621–2628. doi: 10.3892/ol.2017.6525

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Copyright: © Rotoli et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Confocal microscopic analysis of (A) IQGAP1 (green) and (B) β-tubulin (red) expression by double immunofluorescence staining on CRC tissue sections. (C) IQGAP1 and β-tubulin colocalization and with (D) the addition of the nuclear stain DAPI. In the majority of cells, IQGAP1 co-located with microtubules at the cytoplasmic face of the nuclear envelope (arrows). Note that several cells exhibited increased IQGAP1 expression at the plasma membrane (arrowhead) at areas with no (or extremely low) tubulin expression. IQGAP1, IQ-motif containing GTPase activating protein 1.