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. 2017 Jul 8;14(3):2947–2953. doi: 10.3892/ol.2017.6542

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Copyright: © Feng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Rab27A increased the growth of MDA-MB-231 mammospheres. (A) RT-qPCR analysis of the mRNA level of Rab27A following overexpression of Rab27A. (B) Western blot analysis of the protein level of Rab27A following overexpression of Rab27A. (C) RT-qPCR analysis of the mRNA level of Rab27A following interference with Rab27A expression. (D) Western blot analysis of the protein level of Rab27A following interference with Rab27A expression. (E) Following Rab27A overexpression or knockdown, respectively, mammosphere formation by the MDA-MB-231 cell line was observed using a light microscope. (F) FACS demonstrated the proportion of CD44⁺/CD24^−/low cells of the MDA-MB-231 cell line following Rab27A knockdown. (G) Data of (F) represented as the mean ± SD. Error bars=SD between triplicates. *P<0.05 vs. control. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; FACS, fluorescence-activated cell sorting; CD, cluster of differentiation; SD, standard deviation.