Figure 3.

Rab27A overexpression redistributed the cell cycle of breast cancer stem cells. The cell cycles of MDA-MB-231 sphere cells infected with L.V.-Cherry or L.V.-Rab27A were analyzed using (A) RT-qPCR and (B) western blot analysis. The cell cycles of MDA-MB-231 sphere cells infected with scramble siRNA, which was used as a negative control, or siRab27A were analyzed using (C) RT-qPCR and (D) western blot analysis. The expression of CDK4, cyclin D and p27 were revealed following ectopic up or downregulation of Rab27A. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; CDK4, cyclin-dependent kinase 4; p27, cyclin-dependent kinase inhibitor 1B.