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Analysis of DNA synthesized in isolated rat liver mitochondria. (A) Autoradiogram of newly synthesized mtDNA after incubation with [α-32P]dTTP for 30 or 60 min. mtDNA was isolated after the indicated incubation times, digested with AccI at 55°C for 1 h and subjected to electrophoresis through a 1% agarose slab gel. (B) Hybridization of a Southern blot of a similar gel with a probe containing the D-loop sequence.
