Analysis strategy for the identification of circulating endothelial cells by using flow cytometry: immunophenotyping of FSC-H and FSC-A (a), FSC-H and FSC-A (b), SSC-A and CD146 PE-A (c), SSC-A and CD31 FITC-A (d), SSC-A and CD45 APC-Cy7-A (e), and SSC-A and CD34 PE-Cy7-A (f). Gating and quantification of endothelial cells (nonviable cells, platelets, debris, and nonspecific binding) were excluded in a and b. FSC-H, forward scatter height; FSC-A, forward scatter area; SSC-A, side scatter area; PE-A, phycoerythrin area; FITC-A, fluorescein isothiocyanate area; APC-Cy7-A, allophycocyanin-cyanin7; PE-Cy7-A, phycoerythrin area.