Figure 2.

Western blotting and immunoprecipitation of myc–GUS reporter protein with monoclonal anti-c-Myc antibodies. Protein extracts (50 µg) prepared from wild-type and myc–GUS-expressing Arabidopsis cell lines were separated by SDS–PAGE and immunoblotted using a monoclonal anti-c-Myc antibody (left). An asterisk indicates non-specific cross-reaction of the antibody with a protein of 100 kDa present in both control wild-type and transformed cells. The protein extracts (1 mg) were immunaffinity purified on c-Myc IgG beads. The matrix-bound proteins were eluted with c-Myc peptide, separated by SDS–PAGE and subjected to western blotting with a monoclonal anti-c-Myc antibody (right).