Figure 4.

Quantitative measurement of two-hybrid interactions of AKINβ2 with different SnRK1 α-subunits using a β-galactosidase assay. Yeast strain 187 was co-transformed with a pACT2-AKINβ2 prey and either pAS2-AKIN10, pAS2-AKIN11, pAS2-NPK5 or pAS2-SNF1 as bait. Yeast strains carrying the baits AKIN10 and AKIN11 alone were used as self-activation controls. Three colonies selected on minimal SD medium lacking tryptophan and leucine were grown independently in liquid SD medium to mid exponential phase, then the cells were collected for determination of β-galactosidase activity. Bars indicate standard deviations.