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. 2017 Sep 6;37(36):8595–8611. doi: 10.1523/JNEUROSCI.0878-17.2017

Figure 3.

Figure 3.

Loss of PTEN in I-E pairs leads to increased synaptic strength at three of the four connections. A, Schematic of an I-E pair shows the four synaptic connections tested (color-coded arrowheads). B, Representative traces and mean ± SEM values for Control (left traces and circles) and Mutant (right traces and circles) pairs are shown for each of the four connections. Color code matches the connections shown in A. A summary of all values, numbers of neurons tested, and statistical results are in Table 1. C, Bar graph represents the normalized mean ± SEM PSC peak amplitude values for each of the four connections [Mutant pair relative to Control pair (dotted line)]. D, Representative traces indicate the current response to the 500 mm sucrose application used to assay the size of the readily releasable pool of synaptic vesicles at each of the four connections. The traces from Control pairs are in black and those from Mutant pairs are colored to match the schematic in A. E, Bar graph represents the increase in the number of available synaptic vesicles in the three connections that showed increased synaptic strength. Control pair data (n = 20) are indicated by clear bars and Mutant pair data (n = 23) by colored bars to match the schematic in A. F, Representative traces of mEPSC and mIPSC activity. The traces from Control pairs are in black and those from Mutant pairs are colored to match the schematic in A. G, Bar graph represents the increase in the mEPSC frequency at two of the four connections. Control pair data (n = 20) are indicated by clear bars and Mutant pair data (n = 23) by colored bars to match the schematic in A. H, Representative tracing of a Con GABA (blue) and Pten-KO glutamate (yellow) I-E Mutant pair shows VGLUT1 (green punctae) and VGAT (red punctae) immunofluorescence. Scale bar, 25 μm. I, Bar graph represents the increase in the number of VGLUT1 and VGAT punctae on the Pten-KO neurons, and an increase in VGAT punctae on the Con neurons, in I-E pairs. Control pair data (n = 17) are indicated by clear bars and Mutant pair data (n = 19) by colored bars to match the schematic in A. *p < 0.05 (Mann-Whitney test). **p < 0.01 (Mann-Whitney test).