Table 1.
C. elegans transgenes, alleles, and strains
| Transgene | DNA constructs | Genotype | Strain # |
|---|---|---|---|
| sksEx1 | Punc-18-Munc18-1 WT (cDNA) | unc-18 (e81) X | UHN1 |
| sksEx2 | Punc-18-Munc18-1 WT (cDNA) | unc-18 (e81) X | UHN2 |
| sksEx3 | Punc-18-Munc18-1 WT (cDNA) | unc-18 (e81) X | UHN3 |
| sksEx4 | Punc-18-Munc18-1 P335A (cDNA) | unc-18 (e81) X | UHN4 |
| sksEx5 | Punc-18-Munc18-1 P335A (cDNA) | unc-18 (e81) X | UHN5 |
| sksEx6 | Punc-18-Munc18-1 WT::GFP (cDNA) | unc-18 (e81) X | UHN6 |
| sksEx7 | Punc-18-Munc18-1 WT::GFP (cDNA) | unc-18 (e81) X | UHN7 |
| sksEx8 | Punc-18-Munc18-1 P335A::GFP (cDNA) | unc-18 (e81) X | UHN8 |
| sksEx9 | Punc-18-Munc18-1 P335A::GFP (cDNA) | unc-18 (e81) X | UHN9 |
| sksIs1 | Punc-18-Munc18-1 WT::GFP (sksEx6) | UHN10 | |
| sksIs2 | Punc-18-Munc18-1 P335A::GFP (sksEx8) | UHN11 | |
| unc-18(e81)X; Punc-18-Munc18-1 WT::GFP (sksIs1) | UHN12 | ||
| unc-18(e81)X; Punc-18-Munc18-1 P335A::GFP (sksIs2) | UHN13 | ||
| unc-18(md299)X; Punc-18-Munc18-1 WT::GFP (sksIs1) | UHN14 | ||
| unc-18(md299)X; Punc-18-Munc18-1 P335A::GFP (sksIs2) | UHN15 | ||
| sksIs3 | Punc-18-unc-18 WT (cDNA) | unc-18(md299)X; Punc-18-unc-18 WT | UHN16 |
| sksIs4 | Punc-18-unc-18 P334A (cDNA) | unc-18(md299)X; Punc-18-unc-18 P334A | UHN17 |
| unc-13(s69)I; Punc-18-Munc18-1 P335A::GFP (sksIs2) | UHN18 | ||
| unc-13(s69)I; Punc-18(md299)X; Punc-18-unc-18 WT (sksIs3) | UHN19 | ||
| unc-13(s69)I; Punc-18(md299)X; Punc-18-unc-18 P334A (sksIs4) | UHN20 | ||
| unc-18(sks1[P334A + loxP Pmyo-2-GFP; Prps-27-NeoR loxP])X | UHN21 | ||
| unc-18(sks2[P334A + loxP])X | UHN22 | ||
| tom-1(ok285)I; unc-18(sks2)X | UHN23 | ||
| tom-1(ok285)I; unc-13(s69)I | UHN24 | ||
| unc-13(s69)I; unc-18(sks2)X | UHN25 | ||
| tom-1(ok285)I; unc-13(s69)I; unc-18(sks2)X | UHN26 | ||
| unc-18(sks2)X; unc-49(e407)III | UHN27 |